Association of high-risk human papillomavirus with ocular surface squamous neoplasia: a case-control study in Mexico.

Abstract

Abstract Objective: To investigate the association of high-risk hu-man papilloma virus (HR-HPV) and other risk factors with ocular surface squamous cell neoplasia (OSSN).

Materials and methods: We obtained DNA from 22 fresh frozen OSSN tissues and 22 pterygia as controls, we used a broad-spectrum HPV DNA amplification short PCR fragment to identify HPV infection in all specimens and then genotyped HPV by a reverse hybridization line probe assay. We also obtained demographic, sun exposure, and tobacco consump-tion information.

Results: HR-HPV frequency was 40.9 in the OSSN group and 4.5 in the pterygia group (p0.009). After covariate adjustment, OSSN was associated with HR-HPV (OR16.3, 95CI1.2,218.1, p0.03) and sunburn (OR10.8, 95CI1.8,86.0, p0.02).

Conclusions: Ocular surface squamous cell neoplasia is a multifactorial disease. The strong association between HR-HPV and OSSN, suggests that HR-HPV could play an etiological role in OSSN development.