Protective effect of house screening against indoor Aedes aegypti in Mérida, Mexico: A cluster randomised controlled trial

Abstract

Objective: To evaluate the protective effect of house screening (HS) on indoor Aedes aegypti infestation, abundance and arboviral infection in Merida, Mexico.

Methods: In 2019, we performed a cluster randomised controlled trial (6 control and 6 intervention areas: 100 householdsarea). Intervention clusters received permanently fixed fiberglass HS on all windows and doors. The study included two cross-sectional entomologic surveys, one baseline (dry season in May 2019) and one post-intervention (PI, rainy season between September and October 2019). The presence and number of indoor Aedes females and blood-fed females (indoor mosquito infestation) as well as arboviral infections with dengue (DENV) and Zika (ZIKV) viruses were evaluated in a subsample of 30 houses within each cluster.

Results: HS houses had significantly lower risk for having Aedes aegypti female mosquitoes (odds ratio [OR] 0.56, 95 CI 0.33-0.97, p 0.04) and blood-fed females (OR 0.53, 95 CI 0.28-0.97, p 0.04) than unscreened households from the control arm. Compared to control houses, HS houses had significantly lower indoor Ae. aegypti abundance (rate ratio [RR] 0.50, 95 CI 0.30-0.83, p 0.01), blood-fed Ae. aegypti females (RR 0.48, 95 CI 0.27-0.85, p 0.01) and female Ae. aegypti positive for arboviruses (OR 0.29, 95 CI 0.10-0.86, p 0.02). The estimated intervention efficacy in reducing Ae. aegypti arbovirus infection was 71.

Conclusions: These results provide evidence supporting the use of HS as an effective pesticide-free method to control house infestations with Aedes aegypti and reduce the transmission of Aedes-transmitted viruses such as DENV, chikungunya (CHIKV) and ZIKV.