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Please use this identifier to cite or link to this item: http://repositorio.insp.mx:8080/jspui/handle/20.500.12096/8394
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DC FieldValueLanguage
dc.coverage.spatialnacional-
dc.creatorHernández López, Rubí Gisela-
dc.date.accessioned2024-04-29T22:41:58Z-
dc.date.available2024-04-29T22:41:58Z-
dc.date.issued2021-
dc.identifier.issn1932-6203-
dc.identifier.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8294492/pdf/pone.0254946.pdf-
dc.identifier.urihttps://doi.org/10.1371/journal.pone.0254946.-
dc.identifier.urihttp://repositorio.insp.mx:8080/jspui/handle/20.500.12096/8394-
dc.description.abstractAbstract Introduction: Urine self-sampling for human papillomavirus (HPV)-based cervical cancer screening is a non-invasive method that offers several logistical advantages and high acceptability, reducing barriers related to low screening coverage. This study developed and evaluated the performance of a low-cost urine self-sampling method for HPV-testing and explored the acceptability and feasibility of potential implementation of this alternative in routine screening. Methods: A series of sequential laboratory assays examined the impact of several pre-analytical conditions for obtaining DNA from urine and subsequent HPV detection. Initially, we assessed the effect of ethylaminediaminetetraacetic acid (EDTA) as a DNA preservative examining several variables including EDTA concentration, specimen storage temperature, time between urine collection and DNA extraction, and first-morning micturition versus convenience sample collection. We further evaluated the agreement of HPV-testing between urine and clinician-collected cervical samples among 95 women. Finally, we explored the costs of self-sampling supplies as well as the acceptability and feasibility of urine self-sampling among women and healthcare workers. Results: Our results revealed higher DNA concentrations were obtained when using a 40mM EDTA solution, storing specimens at 25C and extracting DNA within 72 hrs. of urine collection, regardless of using first-morning micturition or a convenience sampling. We observed good agreement (Kappa 0.72) between urine and clinician-collected cervical samples for HPV detection. Furthermore, urine self-sampling was an affordable method (USD 1.10), well accepted among cervical cancer screening users, healthcare workers, and decision-makers. Conclusion: These results suggest urine self-sampling is feasible and appropriate alternative for HPV-testing in HPV-based screening programs in lower-resource contexts.-
dc.formatpdf-
dc.languageeng-
dc.publishernan-
dc.rightsinfo:eu-repo/semantics/openAccess-
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0-
dc.subjectAdult Alphapapillomavirus* / genetics Alphapapillomavirus* / metabolism Cervix Uteri / metabolism Cervix Uteri / virology DNA, Viral* / genetics DNA, Viral* / urine Early Detection of Cancer* Female Humans Middle Aged Papillomavirus Infections* / diagnosis Papillomavirus Infections* / genetics Papillomavirus Infections* / urine Papillomavirus Infections* / virology Urine Specimen Collection* Uterine Cervical Neoplasms* / diagnosis Uterine Cervical Neoplasms* / urine Uterine Cervical Neoplasms* / virology Substances nan-
dc.titlePerformance of an affordable urine self-sampling method for human papillomavirus detection in Mexican women-
dc.typeinfo:eu-repo/semantics/article-
dc.subject.ctiinfo:eu-repo/classification/cti/3-
dc.creator.orcidorcid/0000-0002-5809-1377;Hernández López, Rubí Gisela-
dc.creator.orcidorcid/0000-0001-9593-4946;Attila, L?rincz-
dc.creator.orcidorcid/0000-0002-5471-9614;Hermosillo, Luis-
dc.creator.orcidorcid/0000-0002-5809-1377;Hernández López, Rubí Gisela-
dc.creator.orcidorcid/0000-0003-1259-6369;LAZCANO PONCE, EDUARDO CESAR-
dc.creator.orcidorcid/0000-0003-2106-206X;León-Maldonado, Leith-
dc.creator.orcidorcid/0000-0001-8711-4577;nedjai, belinda-
dc.creator.orcidorcid/0000-0002-9845-024X;Rivera-Paredez, Berenice-
dc.creator.orcidorcid/0000-0002-5113-299X;Salmerón, Jorge-
dc.creator.orcidorcid/0000-0002-4086-2436;Torres-Ibarra, Leticia-
dc.creator.orcidorcid/0000-0003-4515-0777;Velázquez-Cruz, Rafael-
dc.creator.orcidorcid/0000-0003-3229-5846;Wheeler, Cosette-
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