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Please use this identifier to cite or link to this item: http://repositorio.insp.mx:8080/jspui/handle/20.500.12096/8023
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DC FieldValueLanguage
dc.coverage.spatialnacional
dc.creatorSahoo, Malaya
dc.date.accessioned2022-02-16T04:24:32Z-
dc.date.available2022-02-16T04:24:32Z-
dc.date.issued2019
dc.identifier.urisicabi.insp.mx:2019-None
dc.identifier.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6320388/pdf/main.pdf
dc.identifier.urihttps://www.doi.org/10.1016/j.jviromet.2018.11.007
dc.identifier.urihttp://repositorio.insp.mx:8080/jspui/handle/20.500.12096/8023-
dc.description.abstractPolioviruses are members of the Enterovirus C species and asymptomatic fecal shedding allows for their transmission and persistence in a community, as well as the emergence of vaccine-derived polioviruses. Using three serotype-specific real-time RT-PCR (rRT-PCR) assays, the shedding and circulation of oral poliovirus vaccine (OPV) strains was previously investigated in a prospective cohort of Mexican children, their contacts, and nearby sewage. Subsequently, a deep sequencing approach targeting the P1 genomic region was applied to characterize OPV strains previously detected by rRT-PCR. Amplifiable RNA was obtained for sequencing from 40.3% (58/144) of stool samples and 71.4% (15/21) of sewage using nucleic acids extracted directly from primary rRT-PCR-positive specimens. Sequencing detected one or more OPV serotypes in 62.1% (36/58) of stool and 53.3% (8/15) of sewage samples. All stool and sewage samples in which poliovirus was not detected by deep sequencing contained at least one non-polio enterovirus C (NPEV-C) strain. To improve screening specificity, a modified, two-step, OPV serotype-specific multiplex rRT-PCR was evaluated. In stool specimens, the overall agreement between the original assays and the multiplex was 70.3%. By serotype, the overall agreement was 95.7% for OPV serotype-1 (S1), 65.6% for S2, and 96.1% for S3. Furthermore, most original rRT-PCR positive/multiplex rRT-PCR negative results were collected in the summer and fall months, consistent with NPEV-C circulation patterns. In conclusion, this deep sequencing approach allowed for the characterization of OPV sequences directly from clinical samples and facilitated the implementation of a more specific multiplex rRT-PCR for OPV detection and serotyping.
dc.formatpdf
dc.languagespa
dc.publisherESPM INSP
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.subjectEnterovirus C, Human geneticsEnterovirus C, Human isolation purificationFeces virologyHigh-Throughput Nucleotide Sequencing,HumansPoliovirus geneticsPoliovirus isolation purification,Prospective StudiesRNA, Viral geneticsReal-Time Polymerase Chain ReactionReverse Transcriptase Polymerase Chain Reaction methodsSensitivity and SpecificitySerogroupSewage virology,SD
dc.titleDeep sequencing prompts the modification of a real-time RT-PCR for the serotype-specific detection of polioviruses
dc.typeinfo:eu-repo/semantics/article
dc.subject.ctiinfo:eu-repo/classification/cti/3
dc.creator.orcidorcid/0000-0001-9837-4032;Sahoo, Malaya
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