Detection of Helicobacter pylori DNA in purified water for drinking

Abstract

H. pylori is a Gram negative bacterium microaerophilic that is associated to digestive human diseases such as gastritis, peptic ulcer, mucosa associated lymphoid tissue lymphoma; this bacterium is considered a risk factor in the development of gastric cancer. Different vias have been proposed by transmission of H. pylori. H. pylori has been detected in well water, municipal water, treated wastewater drinking water in developing countries. The present study shows an evidence of the occurrence of H. pylori through detection of DNA of this bacterium in purified water for drinking of Puebla city. In this work we apply nested PCR to the detection of H. pylori using primers that specifically amplify the gene 16S rRNA